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  • David Hui, PhD
  • Professor, Vice-Chair of Research
  •  
  • Metabolic Diseases Institute Room 257
  • Phone: (513) 558-9152
  • david.hui@uc.edu

Specialty/Interest:

  • Lipid Metabolism, Atherosclerosis, Diabetes, Obesity, Vascular Biology

    Our research program focuses on three specific areas relating cholesterol metabolism with individual susceptibility for coronary heart disease.

    Particular emphasis is placed on the genetic factors that are important for regulation of cholesterol transport and in the maintenance of cell functions in the arterial wall.

    One area of research uses induced mutant mice (transgenic and knockout mice) to determine the role of lipolytic enzymes and intestinal cell surface transporters in mediating dietary fat and cholesterol absorption through the gastrointestinal tract.

    Mutant mice generated from these studies are also being used as an animal model to explore the potential of gene therapy as treatment for nutrient alabsorption due to pancreatic insufficiency. The mechanism by which the lipolytic enzymes and transport proteins mediates cholesterol absorption is being explored using protein chemistry and site specific mutagenesis approaches.

    The second area of research uses tissue cell culture and transgenic mice to identify the interactive effects between diet and genetic factors in the maintenance of plasma cholesterol level and determination of atherosclerosis risk.

    The current emphasis is focused on the role of cholesterol esterase, apolipoprotein (apo) E, LDL receptor, HMG-CoA reductase, and other hepatic lipoprotein receptors in determining individual susceptibility to diet-induced hyperlipidemia and atherosclerosis.

    Major effort in the laboratory is also spent on the third project, which is to determine the molecular and cellular events in the arterial wall in response to injury, such as those observed in human subjects undergoing balloon angioplasty.

    A mouse model of arterial injury has been established in the laboratory for this purpose. This model will be used to explore cell signaling mechanisms of arterial smooth muscle cell hyperplasia. Genetic factors that may contribute to or limit the vascular cell responses after injury will be identified by genetic approaches.

    Particular emphasis is placed on testing the hypothesis that apoE has cytostatic functions in the vessel wall, limiting arterial smooth muscle cell hyperplasia after injury, and that apoE gene transfer to the vasculature may be a potential therapeutic treatment to reduce the risk of restenosis after balloon angioplasty.  

    Education/Credentials:

  • Doctorate: Indiana University
  • Fellowship: University of San Fransisco

Post Graduate Training/Education:

  • FellowshipUniversity of San Fransisco, San Fransisco, CA

Research Grants:

  • 2 U24 DK059630-06 National Institute of Diabetes and Digestive and Kidney Diseases. Cincinnati Mouse Metabolic Phenotyping Center, Collaborator, 000033-001.
  • R01 HL065915 National Heart, Lung and Blood Institute. Lipogenesis and Lipoprotein Complications of HIV Therapy, PI, 000078-001.
  • R01-DK-069967 National Institute of Diabetes and Digestive and Kidney Diseases. Phospholipase A2 in Insulin Resistance and Obesity, PI, 001816-001.
  • R01 DK074932 National Institute of Diabetes and Digestive and Kidney Diseases. Role of Lipoprotein Receptors in Diet-Induced Obesity and Diabetes, PI, 002552-001.
  • 5-R01-HL-054176-02-A0-S0-E0 National Heart, Lung and Blood Institute. Dietary PUFA Effects on LCAT Reactivity, PI, 003085-001.
  • 2-R01-HL-61332 National Heart, Lung and Blood Institute. Apolipopprotein E: Impact on Vascular Cell Functions, PI, 003124-001.
  • American Heart Association - National Chapter. HSPG Perlecan Mediates apoE's Anti-proliferative Effect, PI, 003247-001.
  • R01 DK067416 National Institute of Diabetes and Digestive and Kidney Diseases. Role of Lipolytic Enzymes in Intestinal Lipid Transport, PI, 003432-001.
  • Ilypsa, Inc.. Lipid Metabolism Studies, PI, 003523-001.
  • 5-R01-HL-65915-05-A0-S0-E0 National Heart, Lung and Blood Institute. Lipogenesis and Lipoprotein Complications of HIV Therapy, PI, 003694-001.
  • 5-R01-HL-66246-04-A0-S0-E0 National Heart, Lung and Blood Institute. Vascular Lipolysis: A Determinant of Atherosclerosis, PI, 003901-001.
  • Astra Hassle AB-01-A0-S0-E0 A.B. Hassle (Sweden). Inhibitors of Cholesterol Absorption, PI, 003927-001.
  • 5-R01-DK-40917-09-A0-S0-E0 National Institute of Diabetes and Digestive and Kidney Diseases. Cholesterol Esterase in Dietary Lipid Absorption, PI, 004225-001.
  • 5-P01-DK-54504-05-A0-S0-E0 National Institute of Diabetes and Digestive and Kidney Diseases. Molecular Mechanisms of Cholesterol Absorption, PI, 004449-001.
  • HASSLE-02-01-A0-S1-E1 A.B. Hassle (Sweden). Inhibitors of Cholesterol Absorption, PI, 004570-001.
  • 5-R01-HL-61332-04-A0-S0-E0 National Heart, Lung and Blood Institute. Apolipoprotein E: Impact on Vascular Cell Functions, PI, 004636-001.
  • R01 DK076907 National Institute of Diabetes and Digestive and Kidney Diseases. Physiological Role of NPC1L1 in Lipid Transport and Metabolism, PI, 005026-001.
  • R56 HD034089 National Institute of Child Health and Human Development. Sterol Metabolism During Pregnancy and Development, Collaborator, 005802-001.
  • U01 CA141464 National Cancer Institute. The Effect of Diet and Nutrients on the Progression and Treatment of Prostate Cancer, Collaborator, 006477-001.
  • 3 R01 DK069967-04S2 National Institute of Diabetes and Digestive and Kidney Diseases. ARRA (R01 DK069967) Equipment Request for Phospholipase A2 in Insulin Resistance and Obesity, PI, 006678-001.
  • R01 HL076684 National Heart, Lung and Blood Institute. Angiotensin II, Oxidative Stress and Aneurysm Formation, Collaborator, 006727-001.
  • R01 DK074932 National Institute of Diabetes and Digestive and Kidney Diseases. LDL Receptor Related Protein-1 in Metabolic and Cardiovascular Disease Modulation, PI, 007379-001.
  • R01 DK069967 National Institute of Diabetes and Digestive and Kidney Diseases. Role of Group 1B Phospholipase A2 on Diet-Induced Metabolic Diseases, PI, 007398-001.

Peer Reviewed Publications (in chronological order):

  • Basford, J. E., Wancata, L., Hofmann, S. M., Silva, R. A. G. D., Davidson, W. S., Howles, P. N., & Hui, D. Y. (2011). Hepatic Deficiency of Low Density Lipoprotein Receptor-related Protein-1 Reduces High Density Lipoprotein Secretion and Plasma Levels in Mice . The Journal of Biological Chemistry , 286 (15) , 13079-87.
  • Adams, M. R., Konaniah, E., Cash, J. G., & Hui, D. Y. (2011). Use of NBD-cholesterol to identify a minor but NPC1L1-independent cholesterol absorption pathway in mouse intestine . American Journal of Physiology. Gastrointestinal and Liver Physiology , 300 (1) , G164-9.
  • Yang, L., Li, X., Ji, Y., Kohan, A. B., Wang, D. Q., Howles, P. N., Hui, D. Y., Lai, J., & Tso, P. (2010). Effect of ezetimibe on incretin secretion in response to the intestinal absorption of a mixed meal . American Journal of Physiology. Gastrointestinal and Liver Physiology , 299 (5) , G1003-11.
  • Cash, J. G., Kuhel, D. G., Goodin, C., & Hui, D. Y. (2010). Pancreatic acinar cell-specific overexpression of group 1B phospholipase A(2) exacerbates diet-induced obesity and insulin resistance in mice . International Journal of Obesity (2005).
  • Labonté, E. D., Pfluger, P. T., Cash, J. G., Kuhel, D. G., Roja, J. C., Magness, D. P., Jandacek, R. J., Tschöp, M. H., & Hui, D. Y. (2010). Postprandial lysophospholipid suppresses hepatic fatty acid oxidation: the molecular link between group 1B phospholipase A2 and diet-induced obesity . The FASEB Journal : Official Publication of the Federation of American Societies For Experimental Biology , 24 (7) , 2516-24.
  • Chambliss, K. L., Wu, Q., Oltmann, S., Konaniah, E. S., Umetani, M., Korach, K. S., Thomas, G. D., Mineo, C., Yuhanna, I. S., Kim, S. H., Madak-Erdogan, Z., Maggi, A., Dineen, S. P., Roland, C. L., Hui, D. Y., Brekken, R. A., Katzenellenbogen, J. A., Katzenellenbogen, B. S., & Shaul, P. W. (2010). Non-nuclear estrogen receptor alpha signaling promotes cardiovascular protection but not uterine or breast cancer growth in mice . The Journal of Clinical Investigation , 120 (7) , 2319-30.
  • Perez-Tilve, D., Hofmann, S. M., Basford, J., Nogueiras, R., Pfluger, P. T., Patterson, J. T., Grant, E., Wilson-Perez, H. E., Granholm, N. A., Arnold, M., Trevaskis, J. L., Butler, A. A., Davidson, W. S., Woods, S. C., Benoit, S. C., Sleeman, M. W., DiMarchi, R. D., Hui, D. Y., & Tschöp, M. H. (2010). Melanocortin signaling in the CNS directly regulates circulating cholesterol . Nature Neuroscience , 13 (7) , 877-82.
  • Nawrocki, A. R., Hofmann, S. M., Teupser, D., Basford, J. E., Durand, J. L., Jelicks, L. A., Woo, C. W., Kuriakose, G., Factor, S. M., Tanowitz, H. B., Hui, D. Y., Tabas, I., & Scherer, P. E. (2010). Lack of association between adiponectin levels and atherosclerosis in mice . Arteriosclerosis, Thrombosis, and Vascular Biology , 30 (6) , 1159-65.
  • Qin, Z., Konaniah, E. S., Neltner, B., Nemenoff, R. A., Hui, D. Y., & Weintraub, N. L. (2010). Participation of ATP7A in macrophage mediated oxidation of LDL . Journal of Lipid Research , 51 (6) , 1471-7.
  • Zhu, Y., Kodvawala, A., & Hui, D. Y. (2010). Apolipoprotein E inhibits toll-like receptor (TLR)-3- and TLR-4-mediated macrophage activation through distinct mechanisms . The Biochemical Journal , 428 (1) , 47-54.
  • Basford, J. E., Moore, Z. W. Q., Zhou, L., Herz, J., & Hui, D. Y. (2009). Smooth muscle LDL receptor-related protein-1 inactivation reduces vascular reactivity and promotes injury-induced neointima formation . Arteriosclerosis, Thrombosis, and Vascular Biology , 29 (11) , 1772-8.
  • Hui, D. Y., Cope, M. J., Labonté, E. D., Chang, H., Shao, J., Goka, E., Abousalham, A., Charmot, D., & Buysse, J. (2009). The phospholipase A(2) inhibitor methyl indoxam suppresses diet-induced obesity and glucose intolerance in mice . British Journal of Pharmacology , 157 (7) , 1263-9.
  • Pinkaew, D., Cho, S. G., Hui, D. Y., Wiktorowicz, J. E., Hutadilok-Towatana, N., Mahabusarakam, W., Tonganunt, M., Stafford, L. J., Phongdara, A., Liu, M., & Fujise, K. (2009). (In Press). Morelloflavone blocks injury-induced neointimal formation by inhibiting vascular smooth muscle cell migration. . Biochimica Et Biophysica Acta , 1790 (1) , 31-9.
  • Labonté, E. D., Camarota, L. M., Rojas, J. C., Jandacek, R. J., Gilham, D. E., Davies, J. P., Ioannou, Y. A., Tso, P., Hui, D. Y., & Howles, P. N. (2008). (In Press). Reduced absorption of saturated fatty acids and resistance to diet-induced obesity and diabetes by ezetimibe-treated and Npc1l1-/- mice. . American Journal of Physiology. Gastrointestinal and Liver Physiology , 295 (4) , G776-83.
  • Faulkner, L. E., Panagotopulos, S. E., Johnson, J. D., Woollett, L. A., Hui, D. Y., Witting, S. R., Maiorano, J. N., & Davidson, W. S. (2008). (In Press). An analysis of the role of a retroendocytosis pathway in ABCA1-mediated cholesterol efflux from macrophages. . Journal of Lipid Research , 49 (6) , 1322-32.
  • Hui, D. Y., Labonté, E. D., & Howles, P. N. (2008). (In Press). Development and physiological regulation of intestinal lipid absorption. III. Intestinal transporters and cholesterol absorption. . American Journal of Physiology. Gastrointestinal and Liver Physiology , 294 (4) , G839-43.
  • Hui, David Y (2008). (In Press). Intimal hyperplasia in murine models. . Current Drug Targets , 9 (3) , 251-60.
  • Hofmann, S. M., Perez-Tilve, D., Greer, T. M., Coburn, B. A., Grant, E., Basford, J. E., Tschöp, M. H., & Hui, D. Y. (2008). (In Press). Defective lipid delivery modulates glucose tolerance and metabolic response to diet in apolipoprotein E-deficient mice. . Diabetes , 57 (1) , 5-12.
  • Hofmann, S. M., Zhou, L., Perez-Tilve, D., Greer, T., Grant, E., Wancata, L., Thomas, A., Pfluger, P. T., Basford, J. E., Gilham, D., Herz, J., Tschöp, M. H., & Hui, D. Y. (2007). (In Press). Adipocyte LDL receptor-related protein-1 expression modulates postprandial lipid transport and glucose homeostasis in mice. . The Journal of Clinical Investigation , 117 (11) , 3271-82.
  • Nogueiras, R., Wiedmer, P., Perez-Tilve, D., Veyrat-Durebex, C., Keogh, J. M., Sutton, G. M., Pfluger, P. T., Castaneda, T. R., Neschen, S., Hofmann, S. M., Howles, P. N., Morgan, D. A., Benoit, S. C., Szanto, I., Schrott, B., Schürmann, A., Joost, H., Hammond, C., Hui, D. Y., Woods, S. C., Rahmouni, K., Butler, A. A., Farooqi, I. S., O'Rahilly, S., Rohner-Jeanrenaud, F., & Tschöp, M. H. (2007). (In Press). The central melanocortin system directly controls peripheral lipid metabolism. . The Journal of Clinical Investigation , 117 (11) , 3475-88.
  • Labonté, E. D., Howles, P. N., Granholm, N. A., Rojas, J. C., Davies, J. P., Ioannou, Y. A., & Hui, D. Y. (2007). (In Press). Class B type I scavenger receptor is responsible for the high affinity cholesterol binding activity of intestinal brush border membrane vesicles. . Biochimica Et Biophysica Acta , 1771 (9) , 1132-9.
  • Kim, J., van de Wall, E., Laplante, M., Azzara, A., Trujillo, M. E., Hofmann, S. M., Schraw, T., Durand, J. L., Li, H., Li, G., Jelicks, L. A., Mehler, M. F., Hui, D. Y., Deshaies, Y., Shulman, G. I., Schwartz, G. J., & Scherer, P. E. (2007). (In Press). Obesity-associated improvements in metabolic profile through expansion of adipose tissue. . The Journal of Clinical Investigation , 117 (9) , 2621-37.
  • Gilham, D., Labonté, E. D., Rojas, J. C., Jandacek, R. J., Howles, P. N., & Hui, D. Y. (2007). (In Press). Carboxyl ester lipase deficiency exacerbates dietary lipid absorption abnormalities and resistance to diet-induced obesity in pancreatic triglyceride lipase knockout mice. . The Journal of Biological Chemistry , 282 (34) , 24642-9.
  • Hui, David Y (2007). (In Press). A no-no for NonO and JNK in extracellular matrix homeostasis and vascular stability. . Arteriosclerosis, Thrombosis, and Vascular Biology , 27 (8) , 1677-8.
  • Frolov, A., & Hui, D. Y. (2007). (In Press). The modern art of atherosclerosis: a picture of colorful plants, cholesterol, and inflammation. . Arteriosclerosis, Thrombosis, and Vascular Biology , 27 (3) , 450-2.
  • Tschöp, M. H., Hui, D. Y., & Horvath, T. L. (2007). (In Press). Diet-induced leptin resistance: the heart of the matter. . Endocrinology , 148 (3) , 921-3.
  • Ting, H. J., Stice, J. P., Schaff, U. Y., Hui, D. Y., Rutledge, J. C., Knowlton, A. A., Passerini, A. G., & Simon, S. I. (2007). (In Press). Triglyceride-rich lipoproteins prime aortic endothelium for an enhanced inflammatory response to tumor necrosis factor-alpha. . Circulation Research , 100 (3) , 381-90.
  • Labonté, E. D., Kirby, R. J., Schildmeyer, N. M., Cannon, A. M., Huggins, K. W., & Hui, D. Y. (2006). (In Press). Group 1B phospholipase A2-mediated lysophospholipid absorption directly contributes to postprandial hyperglycemia. . Diabetes , 55 (4) , 935-41.
  • Davidson, W. S., Ghering, A. B., Beish, L., Tubb, M. R., Hui, D. Y., & Pearson, K. (2006). (In Press). The biotin-capture lipid affinity assay: a rapid method for determining lipid binding parameters for apolipoproteins. . Journal of Lipid Research , 47 (2) , 440-9.
  • Kodvawala, A., Ghering, A. B., Davidson, W. S., & Hui, D. Y. (2005). (In Press). Carboxyl ester lipase expression in macrophages increases cholesteryl ester accumulation and promotes atherosclerosis. . The Journal of Biological Chemistry , 280 (46) , 38592-8.
  • Moore, Z. W. Q., & Hui, D. Y. (2005). (In Press). Apolipoprotein E inhibition of vascular hyperplasia and neointima formation requires inducible nitric oxide synthase. . Journal of Lipid Research , 46 (10) , 2083-90.
  • Pham, T., Kodvawala, A., & Hui, D. Y. (2005). (In Press). The receptor binding domain of apolipoprotein E is responsible for its antioxidant activity. . Biochemistry , 44 (20) , 7577-82.
  • Hui, D. Y., & Howles, P. N. (2005). (In Press). Molecular mechanisms of cholesterol absorption and transport in the intestine. . Seminars in Cell & Developmental Biology , 16 (2) , 183-92.
  • Hui, D. Y., & Basford, J. E. (2005). (In Press). Distinct signaling mechanisms for apoE inhibition of cell migration and proliferation. . Neurobiology of Aging , 26 (3) , 317-23.
  • Hui, David Y (2004). (In Press). Apolipoprotein E-induced cell signaling in the vessel wall. . Reviews in Endocrine & Metabolic Disorders , 5 (4) , 335-41.
  • Camarota, L. M., Chapman, J. M., Hui, D. Y., & Howles, P. N. (2004). (In Press). Carboxyl ester lipase cofractionates with scavenger receptor BI in hepatocyte lipid rafts and enhances selective uptake and hydrolysis of cholesteryl esters from HDL3. . The Journal of Biological Chemistry , 279 (26) , 27599-606.
  • Moore, Z. W. Q., Zhu, B., Kuhel, D. G., & Hui, D. Y. (2004). (In Press). Vascular apolipoprotein e expression and recruitment from circulation to modulate smooth muscle cell response to endothelial denudation. . The American Journal of Pathology , 164 (6) , 2109-16.
  • Herz, J., & Hui, D. Y. (2004). (In Press). Lipoprotein receptors in the vascular wall. . Current Opinion in Lipidology , 15 (2) , 175-81.
  • Kirby, R. J., Howles, P. N., & Hui, D. Y. (2004). (In Press). Rate of gastric emptying influences dietary cholesterol absorption efficiency in selected inbred strains of mice. . Journal of Lipid Research , 45 (1) , 89-98.
  • Huggins, K. W., Camarota, L. M., Howles, P. N., & Hui, D. Y. (2003). (In Press). Pancreatic triglyceride lipase deficiency minimally affects dietary fat absorption but dramatically decreases dietary cholesterol absorption in mice. . The Journal of Biological Chemistry , 278 (44) , 42899-905.
  • Zhu, Y., & Hui, D. Y. (2003). (In Press). Apolipoprotein E binding to low density lipoprotein receptor-related protein-1 inhibits cell migration via activation of cAMP-dependent protein kinase A. . The Journal of Biological Chemistry , 278 (38) , 36257-63.
  • Riddle, T. M., Fichtenbaum, C. J., & Hui, D. Y. (2003). (In Press). Leptin replacement therapy but not dietary polyunsaturated fatty acid alleviates HIV protease inhibitor-induced dyslipidemia and lipodystrophy in mice. . Journal of Acquired Immune Deficiency Syndromes (1999) , 33 (5) , 564-70.
  • Hui, David Y (2003). (In Press). Effects of HIV protease inhibitor therapy on lipid metabolism. . Progress in Lipid Research , 42 (2) , 81-92.
  • Hui, David Y (2003). (In Press). HIV protease inhibitors and atherosclerosis. . The Journal of Clinical Investigation , 111 (3) , 317-8.
  • Hui, D. Y., & Howles, P. N. (2002). (In Press). Carboxyl ester lipase: structure-function relationship and physiological role in lipoprotein metabolism and atherosclerosis. . Journal of Lipid Research , 43 (12) , 2017-30.
  • Huggins, K. W., Boileau, A. C., & Hui, D. Y. (2002). (In Press). Protection against diet-induced obesity and obesity- related insulin resistance in Group 1B PLA2-deficient mice. . American Journal of Physiology. Endocrinology and Metabolism , 283 (5) , E994-E1001.
  • Panagotopulos, S. E., Witting, S. R., Horace, E. M., Hui, D. Y., Maiorano, J. N., & Davidson, W. S. (2002). (In Press). The role of apolipoprotein A-I helix 10 in apolipoprotein-mediated cholesterol efflux via the ATP-binding cassette transporter ABCA1. . The Journal of Biological Chemistry , 277 (42) , 39477-84.
  • Zeleny, M., Swertfeger, D. K., Weisgraber, K. H., & Hui, D. Y. (2002). (In Press). Distinct apolipoprotein E isoform preference for inhibition of smooth muscle cell migration and proliferation. . Biochemistry , 41 (39) , 11820-3.
  • Riddle, T. M., Schildmeyer, N. M., Phan, C., Fichtenbaum, C. J., & Hui, D. Y. (2002). (In Press). The HIV protease inhibitor ritonavir increases lipoprotein production and has no effect on lipoprotein clearance in mice. . Journal of Lipid Research , 43 (9) , 1458-63.
  • Kuhel, D. G., Zhu, B., Witte, D. P., & Hui, D. Y. (2002). (In Press). Distinction in genetic determinants for injury-induced neointimal hyperplasia and diet-induced atherosclerosis in inbred mice. . Arteriosclerosis, Thrombosis, and Vascular Biology , 22 (6) , 955-60.
  • Yao, L., Heubi, J. E., Buckley, D. D., Fierra, H., Setchell, K. D. R., Granholm, N. A., Tso, P., Hui, D. Y., & Woollett, L. A. (2002). (In Press). Separation of micelles and vesicles within lumenal aspirates from healthy humans: solubilization of cholesterol after a meal. . Journal of Lipid Research , 43 (4) , 654-60.
  • Zhu, B., Reardon, C. A., Getz, G. S., & Hui, D. Y. (2002). (In Press). Both apolipoprotein E and immune deficiency exacerbate neointimal hyperplasia after vascular injury in mice. . Arteriosclerosis, Thrombosis, and Vascular Biology , 22 (3) , 450-5.
  • Kirby, R. J., Zheng, S., Tso, P., Howles, P. N., & Hui, D. Y. (2002). (In Press). Bile salt-stimulated carboxyl ester lipase influences lipoprotein assembly and secretion in intestine: a process mediated via ceramide hydrolysis. . The Journal of Biological Chemistry , 277 (6) , 4104-9.
  • Swertfeger, D. K., Bu, G., & Hui, D. Y. (2002). (In Press). Low density lipoprotein receptor-related protein mediates apolipoprotein E inhibition of smooth muscle cell migration. . The Journal of Biological Chemistry , 277 (6) , 4141-6.
  • Riddle, T. M., Kuhel, D. G., Woollett, L. A., Fichtenbaum, C. J., & Hui, D. Y. (2001). (In Press). HIV protease inhibitor induces fatty acid and sterol biosynthesis in liver and adipose tissues due to the accumulation of activated sterol regulatory element-binding proteins in the nucleus. . The Journal of Biological Chemistry , 276 (40) , 37514-9.
  • Zhu, B., Zhao, G., Witte, D. P., Hui, D. Y., & Fagin, J. A. (2001). (In Press). Targeted overexpression of IGF-I in smooth muscle cells of transgenic mice enhances neointimal formation through increased proliferation and cell migration after intraarterial injury. . Endocrinology , 142 (8) , 3598-606.
  • Swertfeger, D. K., & Hui, D. Y. (2001). (In Press). Apolipoprotein E receptor binding versus heparan sulfate proteoglycan binding in its regulation of smooth muscle cell migration and proliferation. . The Journal of Biological Chemistry , 276 (27) , 25043-8.
  • Cai, S. F., Kirby, R. J., Howles, P. N., & Hui, D. Y. (2001). (In Press). Differentiation-dependent expression and localization of the class B type I scavenger receptor in intestine. . Journal of Lipid Research , 42 (6) , 902-9.
  • Richmond, B. L., Boileau, A. C., Zheng, S., Huggins, K. W., Granholm, N. A., Tso, P., & Hui, D. Y. (2001). (In Press). Compensatory phospholipid digestion is required for cholesterol absorption in pancreatic phospholipase A(2)-deficient mice. . Gastroenterology , 120 (5) , 1193-202.
  • Du, H., Heur, M., Duanmu, M., Grabowski, G. A., Hui, D. Y., Witte, D. P., & Mishra, J. (2001). (In Press). Lysosomal acid lipase-deficient mice: depletion of white and brown fat, severe hepatosplenomegaly, and shortened life span. . Journal of Lipid Research , 42 (4) , 489-500.
  • Swertfeger, D. K., & Hui, D. Y. (2001). (In Press). Apolipoprotein E: a cholesterol transport protein with lipid transport-independent cell signaling properties. . Frontiers in Bioscience : A Journal and Virtual Library , 6 , D526-35.
  • Bridges, J. P., Davis, H. W., Damodarasamy, M., Kuroki, Y., Howles, G., Hui, D. Y., & McCormack, F. X. (2000). (In Press). Pulmonary surfactant proteins A and D are potent endogenous inhibitors of lipid peroxidation and oxidative cellular injury. . The Journal of Biological Chemistry , 275 (49) , 38848-55.
  • Zhu, B., Kuhel, D. G., Witte, D. P., & Hui, D. Y. (2000). (In Press). Apolipoprotein E inhibits neointimal hyperplasia after arterial injury in mice. . The American Journal of Pathology , 157 (6) , 1839-48.
  • Kuhel, D. G., Zheng, S., Tso, P., & Hui, D. Y. (2000). (In Press). Adenovirus-mediated human pancreatic lipase gene transfer to rat bile: gene therapy of fat malabsorption. . American Journal of Physiology. Gastrointestinal and Liver Physiology , 279 (5) , G1031-6.
  • Liang, Y., Medhekar, R., Brockman, H. L., Quinn, D. M., & Hui, D. Y. (2000). (In Press). Importance of arginines 63 and 423 in modulating the bile salt-dependent and bile salt-independent hydrolytic activities of rat carboxyl ester lipase. . The Journal of Biological Chemistry , 275 (31) , 24040-6.
  • Keelan, M., Hui, D. Y., Wild, G., Clandinin, M. T., & Thomson, A. B. (2000). (In Press). Variability of the intestinal uptake of lipids is genetically determined in mice. . Lipids , 35 (8) , 833-7.
  • Ishigami, M., Swertfeger, D. K., Hui, M. S., Granholm, N. A., & Hui, D. Y. (2000). (In Press). Apolipoprotein E inhibition of vascular smooth muscle cell proliferation but not the inhibition of migration is mediated through activation of inducible nitric oxide synthase. . Arteriosclerosis, Thrombosis, and Vascular Biology , 20 (4) , 1020-6.
  • Richmond, B. L., & Hui, D. Y. (2000). (In Press). Molecular structure and tissue-specific expression of the mouse pancreatic phospholipase A(2) gene. . Gene , 244 (1-2) , 65-72.
  • Howles, P. N., Stemmerman, G. N., Fenoglio-Preiser, C. M., & Hui, D. Y. (1999). (In Press). Carboxyl ester lipase activity in milk prevents fat-derived intestinal injury in neonatal mice. . The American Journal of Physiology , 277 (3 Pt 1) , G653-61.
  • Young, S. C., & Hui, D. Y. (1999). (In Press). Pancreatic lipase/colipase-mediated triacylglycerol hydrolysis is required for cholesterol transport from lipid emulsions to intestinal cells. . The Biochemical Journal , 339 ( Pt 3) , 615-20.
  • Hui, D Y (1998). (In Press). Utility and importance of gene knockout animals for nutritional and metabolic research. . The Journal of Nutrition , 128 (11) , 2052-7.
  • Ishigami, M., Swertfeger, D. K., Granholm, N. A., & Hui, D. Y. (1998). (In Press). Apolipoprotein E inhibits platelet-derived growth factor-induced vascular smooth muscle cell migration and proliferation by suppressing signal transduction and preventing cell entry to G1 phase. . The Journal of Biological Chemistry , 273 (32) , 20156-61.
  • Qin, X., Swertfeger, D. K., Zheng, S., Hui, D. Y., & Tso, P. (1998). (In Press). Apolipoprotein AIV: a potent endogenous inhibitor of lipid oxidation. . The American Journal of Physiology , 274 (5 Pt 2) , H1836-40.
  • Li, F., & Hui, D. Y. (1998). (In Press). Synthesis and secretion of the pancreatic-type carboxyl ester lipase by human endothelial cells. . The Biochemical Journal , 329 ( Pt 3) , 675-9.
  • Li, F., & Hui, D. Y. (1997). (In Press). Modified low density lipoprotein enhances the secretion of bile salt-stimulated cholesterol esterase by human monocyte-macrophages. species-specific difference in macrophage cholesteryl ester hydrolase. . The Journal of Biological Chemistry , 272 (45) , 28666-71.
  • Hornick, C. A., Hui, D. Y., & DeLamatre, J. G. (1997). (In Press). A role for retrosomes in intracellular cholesterol transport from endosomes to the plasma membrane. . The American Journal of Physiology , 273 (3 Pt 1) , C1075-81.
  • Carter, C. P., Howles, P. N., & Hui, D. Y. (1997). (In Press). Genetic variation in cholesterol absorption efficiency among inbred strains of mice. . The Journal of Nutrition , 127 (7) , 1344-8.
  • Hui, D Y (1997). (In Press). Use of gene knockout mice to establish lipase function. . Methods in Enzymology , 286 , 67-80.
  • Feaster, S. R., Lee, K., Baker, N., Hui, D. Y., & Quinn, D. M. (1996). (In Press). Molecular recognition by cholesterol esterase of active site ligands: structure-reactivity effects for inhibition by aryl carbamates and subsequent carbamylenzyme turnover. . Biochemistry , 35 (51) , 16723-34.
  • Hui, D Y (1996). (In Press). Molecular biology of enzymes involved with cholesterol ester hydrolysis in mammalian tissues. . Biochimica Et Biophysica Acta , 1303 (1) , 1-14.
  • Li, F., Huang, Y., & Hui, D. Y. (1996). (In Press). Bile salt stimulated cholesterol esterase increases uptake of high density lipoprotein-associated cholesteryl esters by HepG2 cells. . Biochemistry , 35 (21) , 6657-63.
  • Howles, P. N., Carter, C. P., & Hui, D. Y. (1996). (In Press). Dietary free and esterified cholesterol absorption in cholesterol esterase (bile salt-stimulated lipase) gene-targeted mice. . The Journal of Biological Chemistry , 271 (12) , 7196-202.
  • Brodt-Eppley, J., White, P., Jenkins, S., & Hui, D. Y. (1995). (In Press). Plasma cholesterol esterase level is a determinant for an atherogenic lipoprotein profile in normolipidemic human subjects. . Biochimica Et Biophysica Acta , 1272 (2) , 69-72.
  • Myers-Payne, S. C., Hui, D. Y., Brockman, H. L., & Schroeder, F. (1995). (In Press). Cholesterol esterase: a cholesterol transfer protein. . Biochemistry , 34 (12) , 3942-7.
  • Brodt-Eppley, J., & Hui, D. Y. (1995). (In Press). Calcium mobilization and protein kinase C activation are required for cholecystokinin stimulation of pancreatic cholesterol esterase secretion. . The Biochemical Journal , 306 ( Pt 2) , 605-8.
  • Huang, Y., & Hui, D. Y. (1994). (In Press). Increased cholesterol esterase level by cholesterol loading of rat pancreatoma cells. . Biochimica Et Biophysica Acta , 1214 (3) , 317-22.
  • Huang, Y., & Hui, D. Y. (1994). (In Press). Synergistic effects of bombesin and cholecystokinin on cholesterol esterase biosynthesis and secretion by AR42J cells. . Archives of Biochemistry and Biophysics , 310 (1) , 54-9.
  • DiPersio, L. P., Carter, C. P., & Hui, D. Y. (1994). (In Press). Exon 11 of the rat cholesterol esterase gene encodes domains important for intracellular processing and bile salt-modulated activity of the protein. . Biochemistry , 33 (11) , 3442-8.
  • Brodt-Eppley, J., & Hui, D. Y. (1994). (In Press). Dietary regulation of cholesterol esterase mRNA level in rat pancreas. . Journal of Lipid Research , 35 (1) , 27-35.
  • Warden, C. H., Davis, R. C., Yoon, M. Y., Hui, D. Y., Svenson, K., Xia, Y. R., Diep, A., He, K. Y., & Lusis, A. J. (1993). (In Press). Chromosomal localization of lipolytic enzymes in the mouse: pancreatic lipase, colipase, hormone-sensitive lipase, hepatic lipase, and carboxyl ester lipase. . Journal of Lipid Research , 34 (8) , 1451-5.
  • Hui, D. Y., Hayakawa, K., & Oizumi, J. (1993). (In Press). Lipoamidase activity in normal and mutagenized pancreatic cholesterol esterase (bile salt-stimulated lipase). . The Biochemical Journal , 291 ( Pt 1) , 65-9.
  • DiPersio, L. P., & Hui, D. Y. (1993). (In Press). Aspartic acid 320 is required for optimal activity of rat pancreatic cholesterol esterase. . The Journal of Biological Chemistry , 268 (1) , 300-4.
  • DiPersio, L. P., Kissel, J. A., & Hui, D. Y. (1992). (In Press). Purification of pancreatic cholesterol esterase expressed in recombinant baculovirus-infected Sf9 cells. . Protein Expression and Purification , 3 (2) , 114-20.
  • Fontaine, R. N., Carter, C. P., & Hui, D. Y. (1991). (In Press). Structure of the rat pancreatic cholesterol esterase gene. . Biochemistry , 30 (28) , 7008-14.
  • Yamashita, S., Hui, D. Y., Wetterau, J. R., Sprecher, D. L., Harmony, J. A., Sakai, N., Matsuzawa, Y., & Tarui, S. (1991). (In Press). Characterization of plasma lipoproteins in patients heterozygous for human plasma cholesteryl ester transfer protein (CETP) deficiency: plasma CETP regulates high-density lipoprotein concentration and composition. . Metabolism: Clinical and Experimental , 40 (7) , 756-63.
  • Huang, Y., & Hui, D. Y. (1991). (In Press). Cholesterol esterase biosynthesis in rat pancreatic AR42J cells. Post-transcriptional activation by gastric hormones. . The Journal of Biological Chemistry , 266 (11) , 6720-5.
  • DiPersio, L. P., Fontaine, R. N., & Hui, D. Y. (1991). (In Press). Site-specific mutagenesis of an essential histidine residue in pancreatic cholesterol esterase. . The Journal of Biological Chemistry , 266 (7) , 4033-6.
  • Hui, D. Y., & Kissel, J. A. (1990). (In Press). Sequence identity between human pancreatic cholesterol esterase and bile salt-stimulated milk lipase. . FEBS Letters , 276 (1-2) , 131-4.
  • Huang, Y., & Hui, D. Y. (1990). (In Press). Metabolic fate of pancreas-derived cholesterol esterase in intestine: an in vitro study using Caco-2 cells. . Journal of Lipid Research , 31 (11) , 2029-37.
  • DiPersio, L. P., Fontaine, R. N., & Hui, D. Y. (1990). (In Press). Identification of the active site serine in pancreatic cholesterol esterase by chemical modification and site-specific mutagenesis. . The Journal of Biological Chemistry , 265 (28) , 16801-6.
  • Yamashita, S., Sprecher, D. L., Sakai, N., Matsuzawa, Y., Tarui, S., & Hui, D. Y. (1990). (In Press). Accumulation of apolipoprotein E-rich high density lipoproteins in hyperalphalipoproteinemic human subjects with plasma cholesteryl ester transfer protein deficiency. . The Journal of Clinical Investigation , 86 (3) , 688-95.
  • Yamashita, S., Hui, D. Y., Sprecher, D. L., Matsuzawa, Y., Sakai, N., Tarui, S., Kaplan, D., Wetterau, J. R., & Harmony, J. A. (1990). (In Press). Total deficiency of plasma cholesteryl ester transfer protein in subjects homozygous and heterozygous for the intron 14 splicing defect. . Biochemical and Biophysical Research Communications , 170 (3) , 1346-51.
  • Kissel, J. A., Fontaine, R. N., Turck, C. W., Brockman, H. L., & Hui, D. Y. (1989). (In Press). Molecular cloning and expression of cDNA for rat pancreatic cholesterol esterase. . Biochimica Et Biophysica Acta , 1006 (2) , 227-36.
  • Camulli, E. D., Linke, M. J., Brockman, H. L., & Hui, D. Y. (1989). (In Press). Identity of a cytosolic neutral cholesterol esterase in rat liver with the bile salt stimulated cholesterol esterase in pancreas. . Biochimica Et Biophysica Acta , 1005 (2) , 177-82.
  • Boyles, J. K., Zoellner, C. D., Anderson, L. J., Kosik, L. M., Pitas, R. E., Weisgraber, K. H., Hui, D. Y., Mahley, R. W., Gebicke-Haerter, P. J., & Ignatius, M. J. (1989). (In Press). A role for apolipoprotein E, apolipoprotein A-I, and low density lipoprotein receptors in cholesterol transport during regeneration and remyelination of the rat sciatic nerve. . The Journal of Clinical Investigation , 83 (3) , 1015-31.
  • Friedman, G., Wernette-Hammond, M. E., Hui, D. Y., Mahley, R. W., & Innerarity, T. L. (1987). (In Press). Characterization of lipoprotein receptors on rat Fu5AH hepatoma cells. . Journal of Lipid Research , 28 (12) , 1482-94.
  • Mahley, R. W., Innerarity, T. L., Hui, D. Y., Weisgraber, K. H., & Pitas, R. E. (1986). (In Press). Pathology: plasma cholesterol levels determined by lipoprotein interactions with cell surface receptors. . The Western Journal of Medicine , 145 (3) , 372.
  • Hui, D. Y., Brecht, W. J., Hall, E. A., Friedman, G., Innerarity, T. L., & Mahley, R. W. (1986). (In Press). Isolation and characterization of the apolipoprotein E receptor from canine and human liver. . The Journal of Biological Chemistry , 261 (9) , 4256-67.
  • Innerarity, T. L., Hui, D. Y., Bersot, T. P., & Mahley, R. W. (1986). (In Press). Type III hyperlipoproteinemia: a focus on lipoprotein receptor-apolipoprotein E2 interactions. . Advances in Experimental Medicine and Biology , 201 , 273-88.
  • Mahley, R. W., Innerarity, T. L., Weisgraber, K. H., Rall, S. C., Hui, D. Y., Lalazar, A., Boyles, J. K., Taylor, J. M., & Levy-Wilson, B. (1986). (In Press). Cellular and molecular biology of lipoprotein metabolism: characterization of lipoprotein receptor-ligand interactions. . Cold Spring Harbor Symposia on Quantitative Biology , 51 Pt 2 , 821-8.
  • Vogel, T., Weisgraber, K. H., Zeevi, M. I., Ben-Artzi, H., Levanon, A. Z., Rall, S. C., Innerarity, T. L., Hui, D. Y., Taylor, J. M., & Kanner, D. (1985). (In Press). Human apolipoprotein E expression in Escherichia coli: structural and functional identity of the bacterially produced protein with plasma apolipoprotein E. . Proceedings of the National Academy of Sciences of the United States of America , 82 (24) , 8696-700.
  • Hui, D. Y., Innerarity, T. L., Milne, R. W., Marcel, Y. L., & Mahley, R. W. (1984). (In Press). Binding of chylomicron remnants and beta-very low density lipoproteins to hepatic and extrahepatic lipoprotein receptors. A process independent of apolipoprotein B48. . The Journal of Biological Chemistry , 259 (24) , 15060-8.
  • Hui, D. Y., Innerarity, T. L., & Mahley, R. W. (1984). (In Press). Defective hepatic lipoprotein receptor binding of beta-very low density lipoproteins from type III hyperlipoproteinemic patients. Importance of apolipoprotein E. . The Journal of Biological Chemistry , 259 (2) , 860-9.
  • Mahley, R. W., Hui, D. Y., Innerarity, T. L., & Weisgraber, K. H. (1981). (In Press). Two independent lipoprotein receptors on hepatic membranes of dog, swine, and man. Apo-B,E and apo-E receptors. . The Journal of Clinical Investigation , 68 (5) , 1197-206.
  • Noel, J. G., Hui, D. Y., Blankenship, D. T., & Harmony, J. A. (1981). (In Press). Reduced immunoregulatory potency of low density lipoproteins with selectively modified arginine and lysine residues of apolipoprotein B. . Biochimica Et Biophysica Acta , 665 (3) , 519-30.
  • Harmony, J. A., & Hui, D. Y. (1981). (In Press). Inhibition by membrane-bound low-density lipoproteins of the primary inductive events of mitogen-stimulated lymphocyte activation. . Cancer Research , 41 (9 Pt 2) , 3799-802.
  • Innerarity, T. L., Kempner, E. S., Hui, D. Y., & Mahley, R. W. (1981). (In Press). Functional unit of the low density lipoprotein receptor of fibroblasts: a 100,000-dalton structure with multiple binding sites. . Proceedings of the National Academy of Sciences of the United States of America , 78 (7) , 4378-82.
  • Hui, D. Y., Noel, J. G., & Harmony, J. A. (1981). (In Press). Binding of plasma low density lipoproteins to erythrocytes. . Biochimica Et Biophysica Acta , 664 (3) , 513-26.
  • Hui, D. Y., Innerarity, T. L., & Mahley, R. W. (1981). (In Press). Lipoprotein binding to canine hepatic membranes. Metabolically distinct apo-E and apo-B,E receptors. . The Journal of Biological Chemistry , 256 (11) , 5646-55.
  • Hui, D. Y., Harmony, J. A., Innerarity, T. L., & Mahley, R. W. (1980). (In Press). Immunoregulatory plasma lipoproteins. Role of apoprotein E and apoprotein B. . The Journal of Biological Chemistry , 255 (24) , 11775-81.
  • Hui, D. Y., & Harmony, J. A. (1980). (In Press). Phosphatidylinositol turnover in mitogen-activated lymphocytes. Suppression by low-density lipoproteins. . The Biochemical Journal , 192 (1) , 91-8.
  • Hui, D. Y., & Harmony, J. A. (1980). (In Press). Inhibition of Ca2+ accumulation in mitogen-activated lymphocytes: role of membrane-bound plasma lipoproteins. . Proceedings of the National Academy of Sciences of the United States of America , 77 (8) , 4764-8.
  • Hui, D. Y., & Harmony, J. A. (1980). (In Press). Inhibition by low density lipoproteins of mitogen-stimulated cyclic nucleotide production by lymphocytes. . The Journal of Biological Chemistry , 255 (4) , 1413-9.
  • Hui, D. Y., Berebitsky, G. L., & Harmony, J. A. (1979). (In Press). Mitogen-stimulated calcium ion accumulation by lymphocytes. Influence of plasma lipoproteins. . The Journal of Biological Chemistry , 254 (11) , 4666-73.
  • Hui, D. Y., & Harmony, J. A. (1979). (In Press). Interaction of plasma lipoproteins with erythrocytes. II. Modulation of membrane-associated enzymes. . Biochimica Et Biophysica Acta , 550 (3) , 425-34.
  • Hui, D. Y., & Harmony, J. A. (1979). (In Press). Interaction of plasma lipoproteins with erythrocytes. I. Alteration of erythrocyte morphology. . Biochimica Et Biophysica Acta , 550 (3) , 407-24.
  • Hui, D. Y., & Harmony, J. A. (1979). (In Press). Erythrocyte spectrin alteration induced by low-density lipoprotein. . Journal of Supramolecular Structure , 10 (2) , 253-63.
  • Mahley, R. W., Hui, D. Y., Innerarity, T. L., & Beisiegel, U. (In Press). Chylomicron remnant metabolism. Role of hepatic lipoprotein receptors in mediating uptake. . Arteriosclerosis (Dallas, Tex.) , 9 (1 Suppl) , I14-8.
  • Beisiegel, U., Weber, W., Havinga, J. R., Ihrke, G., Hui, D. Y., Wernette-Hammond, M. E., Turck, C. W., Innerarity, T. L., & Mahley, R. W. (In Press). Apolipoprotein E-binding proteins isolated from dog and human liver. . Arteriosclerosis (Dallas, Tex.) , 8 (3) , 288-97.
  • Funke, H., Boyles, J., Weisgraber, K. H., Ludwig, E. H., Hui, D. Y., & Mahley, R. W. (In Press). Uptake of apolipoprotein E-containing high density lipoproteins by hepatic parenchymal cells. . Arteriosclerosis (Dallas, Tex.) , 4 (5) , 452-61.